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Equipment

  1. Steady-state absorption and emission spectrometers

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Technique 

Steady-state absorption uses a continuous-wave beam of light to irradiate the sample and the fraction of light absorbed is determined through transmission or reflectance measurements. Steady-state emission techniques measure the electromagnetic radiation emitted upon deactivation of excited states. A series of instruments able to record steady-state absorption, emission and excitation and anisotropy spectra of samples in liquid and solid states. The detection wavelength range spreads from 200 to 1600 nm

Units  

PTI QuantaMaster 40, Horiba Jobin-Yvon Spex Fluorolog-3, Perkin Elmer Lambda 40

Instrument type

commercial

Complexity level

basic

Applications

 

Determining the absorption and emission spectra of a photoactive sample is the first step in the spectroscopic and photophysical characterization of a sample. It also assists in identification and characterization of the molecular entities involved in the processes that are investigated: excited states, intermediates, and photoproducts.