17. SRS Microscope
The method is based on Stimulated Raman Scattering (SRS) in which two beams, pump and Stokes are incident on the sample. When the difference in frequency pump - Stokes matches a molecular vibration, stimulated excitation of the vibrational transition occurs. This process causes an intensity loss in the pump beam) and an intensity gain in the Stokes beam. The change in pump or Stokes beams can be measured via lock-in detection.
Microscope, laser system, lock-in detection.
The setup provides background-free Raman imaging with improved image contrast, both of which are important for biomedical imaging applications.