6. Fluorescence Lifetime Imaging (FLIM)

Technique 

The setup produces an image based on the differences in the excited state decay time of fluorophores accumulated in different regions in a sample. The image contrast is based on the lifetime of individual fluorophores rather than their emission spectra.

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Units

Integrated in SP8 STED

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Instrument type

Commercial

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Complexity level

Medium

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Applications

Fluorescence resonance energy transfer measurements using FLIM can provide a method to discriminate between different fluorophores with strongly overlapping spectra or fluorophores in a different environment.