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Equipment

    6. Fluorescence Lifetime Imaging (FLIM)

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Technique 

The setup produces an image based on the differences in the excited state decay time of fluorophores accumulated in different regions in a sample. The image contrast is based on the lifetime of individual fluorophores rather than their emission spectra.

Units

Integrated in SP8 STED

Instrument type

Commercial

Complexity level

Medium

Applications

Fluorescence resonance energy transfer measurements using FLIM can provide a method to discriminate between different fluorophores with strongly overlapping spectra or fluorophores in a different environment.