7. Single Molecule fluorescence Decay and Spectra on ps-ns time scale

Technique 

The setup monitors the fluorescence decay of a sample at single molecule level via TC-SPC and records the spectra by a combination of a spectrograph and highly sensitive CCD camera detector. For an optimal optical resolution and contrast the fluorescence detection is achieved in a confocal scanning microscope.

​

Units

ILaser system, microscope, detection part.

​

Instrument type

Homemade

​

Complexity level

High

​

Applications

The setup provides fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector. This is important for detecting peculiar processes that averaged at bulk level. The time resolution is limited to 600 ps and spatial resolution to about 300 nm.